MilliporeSigma™ anti-MMP-3 Clone: 55-2A4,

Catalog No.	Quantity	Conjugate
IM36100UG	100 μg	Unconjugated

Catalog No. IM36100UG Supplier MilliporeSigma™ Supplier No. IM36100UG

Description

Mouse Monoclonal Antibody

Specifically detects MMP-3 Clone: 55-2A4 in Human, Porcine, Rat, Sheep samples, and it is validated for Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immuno Blotting

Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-3 (stromelysin-1) is secreted as a 59/57kDa zymogen that is proteolytically processed to the 45 and 28kDa active forms. This enzyme degrades a wide spectrum of extracellular matrix proteins, including cartilage proteoglycans, fibronectin, type IV and IX collagen and laminin. MMP-3 is thought to play an important role in pathophysiological degradation processes associated with conditions such as rheumatoid arthritis and cancer cell invasion.

Specifications

• Antigen: MMP-3
• Applications: Immunoblot, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Classification: Monoclonal
• Clone: 55-2A4
• Conjugate: Unconjugated
• Formulation: In 100mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
• Host Species: Mouse
• Immunogen: human pro-MMP-3 from the conditioned media of rheumatoid synovial fibroblasts
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽57/59kDa latent and the ∽28/45kDa active forms of MMP-3. Will also recognize MMP-3/TIMP-1 complexes. Slightly cross-reacts (∽34%) with human MMP-10.
• Target Species: Human, Pig, Rat, Sheep
• Content And Storage: −20°C
• Form: Purified
• Isotype: IgG1

For Research Use Only