MyoD Antibody (SPM427), DyLight 350, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234772UV

Catalog No. NBP234772UV

Description

MyoD Monoclonal specifically detects MyoD in Human, Mouse, Rat, Chicken samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: MyoD
• Classification: Monoclonal
• Conjugate: DyLight 350
• Gene Alias: bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
• Host Species: Mouse
• Molecular Weight of Antigen: 45 kDa
• Quantity: 0.1 mL
• Research Discipline: Cancer, Cell Cycle and Replication, Growth and Development
• Gene ID (Entrez): 4654
• Target Species: Human, Mouse, Rat, Chicken
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: SPM427
• Dilution: Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Symbols: MYOD1
• Immunogen: Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189. (Uniprot: P15172)
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this monoclonal antibody maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms sarcomas and alveolar soft part sarcomas.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG1 κ

For Research Use Only