anti-TNF-alpha, APC, Clone: SPM543, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234419APC

Catalog No. NBP234419J

Description

TNF-alpha Monoclonal specifically detects TNF-alpha in Human, Mouse, Rat, Canine, Feline, Rabbit, Zebrafish samples. It is validated for Flow Cytometry, Flow (Intracellular).

Specifications

• Antigen: TNF-alpha
• Classification: Monoclonal
• Conjugate: APC
• Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Host Species: Mouse
• Molecular Weight of Antigen: 17 kDa
• Quantity: 0.1 mL
• Research Discipline: Adaptive Immunity, Asthma, Autoimmune Diseases, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
• Gene ID (Entrez): 7124
• Target Species: Human, Mouse, Rat, Canine, Feline, Rabbit, Zebrafish
• Form: Purified

• Applications: Flow Cytometry
• Clone: SPM543
• Dilution: Flow Cytometry, Flow (Intracellular)
• Gene Symbols: TNF
• Immunogen: A hexadecapeptide corresponding to aa115-130 of human TNF-alpha (Uniprot: P01375)
• Purification Method: Protein L or PEG purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgM κ

For Research Use Only