Vimentin Antibody (SPM576), Alexa Fluor™ 488, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234809AF488

Catalog No. NBP234809X

Description

Vimentin Monoclonal specifically detects Vimentin in Human, Porcine, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Vimentin
• Classification: Monoclonal
• Conjugate: Alexa Fluor 488
• Gene Alias: FLJ36605, vimentin
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody reacts with a 58kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of Vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-Vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-Vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain Vimentin, e.g. hepatoma and seminoma. Anti-Vimentin is also useful as a tissue process control reagent.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: SPM576
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Symbols: VIM
• Immunogen: Human vimentin recombinant protein (Uniprot: P08670)
• Quantity: 0.1 mL
• Research Discipline: Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
• Gene ID (Entrez): 7431
• Target Species: Human, Pig, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative)
• Form: Purified

For Research Use Only