MyoD Mouse, Unlabeled, Clone: MOAB 5.8A, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 554130

Catalog No. BDB554130

Description

MyoD is a member of a family of myogenic basic helix-loop-helix (bHLH) transcription factors that includes Myf-5, myogenin, and MRF4 (also known as herculin or Myf-6). Members of this family, expressed exclusively in skeletal muscle, play a key role in myogenesis by activating muscle-specific genes in response to extracellular growth factors. Transfection of MyoD and other family members into non-muscle cells has been shown to either convert these cells to myogenic cells or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. Members of this family have distinct roles in muscle development; for example, MyoD and Myf-5 act early during myeloid development, whereas myogenin acts at a later point during myoblast differentiation. The expression and activity of MyoD has been shown to involve the mitogen-activated protein kinase (MAPK) cascade.
This antibody is routinely tested by western blot analysis. The epitope for MoAb 5.8A has been mapped to amino acids 180-189 of mouse MyoD. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Mouse
Clone: MoAb 5.8A
Isotype: IgG1 κ
Species Reactivity: Human
Immunogen: Recombinant Mouse MyoD
Formula Weight [Chemical]: 45/48kDa

Immunohistochemistry (Frozen), Western Blotting

Specifications

• Antigen: MyoD
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Recombinant Mouse MyoD
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Chicken, Human, Mouse, Rat
• Isotype: IgG1 κ

• Applications: Western Blot
• Clone: MOAB 5.8A
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.