MilliporeSigma™ anti-N-Ethylmaleimide-Sensitive Factor Clone: NSF-1,

Catalog No.	Quantity	Conjugate
NB21100UG	100 μg	Unconjugated

Catalog No. NB21100UG Supplier MilliporeSigma™ Supplier No. NB21100UG

Description

Mouse Monoclonal Antibody

Specifically detects N-Ethylmaleimide-Sensitive Factor Clone: NSF-1 in Human, Rat samples, and it is validated for ELISA, Immunoprecipitation, Immuno Blotting

Synaptic vesicle proteins can be divided into one of two classes; transport proteins involved in neurotransmitter uptake, and trafficking proteins which perform functions in vesicle docking, fusion, and budding. For example, synaptophysin may function in the process of neurotransmitter release, while synaptobrevin (VAMP) is believed to play a role in synaptic vesicle docking and exocytotic membrane fusion. Synaptobrevin has also been shown to bind synaptophysin with high specificity. Synapsins are highly abundant vesicle membrane proteins that are believed to regulate neurotransmitter release through phosphorylation-dependent interactions with cytoskeletal elements. Other proteins involved in vesicle exocytosis include syntaxin 1 and SNAP-25. In conjunction with synaptobrevin, these proteins form the SNARE (SNAP receptor) complex, which recruits other cytosolic factors including SNAP proteins (soluble NSF attachment proteins) and NSF (N-ethylmaleimide-sensitive factor). NSF acts in concert with SNAPs and SNAREs for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicle-to-plasma membrane transport. In its functional form, NSF is a homotrimer of 76kDa subunits. Each subunit contains an N-terminal domain and two homologous ATP binding domains (D1 and D2). Vesicle-to-target membrane docking occurs when the vesicle SNARE binds to its cognate target membrane SNARE. SNAPs α and γ then bind to this docking complex and mediate the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.

Specifications

• Antigen: N-Ethylmaleimide-Sensitive Factor
• Applications: ELISA, Immunoblot, Immunoblot, Immunoprecipitation
• Classification: Monoclonal
• Clone: NSF-1
• Conjugate: Unconjugated
• Formulation: In 150mM NaCl, 20mM sodium phosphate, 50% glycerol, pH 7.5.
• Host Species: Mouse
• Immunogen: recombinant, human N-ethylmaleimide-senstive factor
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽76kDa NSF-1 protein in human fibroblasts, MDBK, and PC12 cells.
• Target Species: Human, Rat
• Content And Storage: −20°C
• Form: Purified
• Isotype: IgG

For Research Use Only