Nestin Mouse, Alexa Fluor 647, Clone: 25/NESTIN, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 560393

Catalog No. BDB560393

Description

The cytoskeleton consists primarily of core structural proteins that include microfilaments, microtubules, and intermediate filaments (IFs). IFs contain more than 50 distinct proteins that are organized into six different subtypes: Type I/II keratins expressed in epithelia, type III vimentin/desmin, type IV neurofilament proteins, type V nuclear lamins, and type VI nestin expressed primarily in embryonic cells. Nestin has a conserved core region (amino acids 7 to 314), which contains an α helical domain that is involved in coiled-coil assembly of IFs. The C-terminal region of nestin is similar to type IV IFs, since it contains highly charged amino acids, many glutamate residues, and an 11 amino acid repeat motif. Nestin is expressed in the cerebrum during embryonic development, in the cerebellum during early postnatal development, and in dermatomal cells and myoblasts during myogenesis. In vitro, nestin forms homodimers and homotetramers, but not IFs, and can co-assemble with type III vimentin and type IV internexin proteins. Thus, nestin is a core IF protein that is essential for proper cytoskeletal formation during neurogenesis and myogenesis.

Specifications

• Antigen: Nestin
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 25/NESTIN
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
• Immunogen: Rat Nestin aa. 402-604 Recombinant Protein
• Quantity: 50 Tests
• Research Discipline: Cell Biology
• Target Species: Human, Rat
• Isotype: IgG1 κ