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Nestin Mouse, Unlabeled, Clone: 25/NESTIN, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 611658

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Catalog No. BDB611658


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Description

Description

The cytoskeleton consists primarily of core structural proteins that include microfilaments, microtubules, and intermediate filaments (IFs). IFs contain more than 50 distinct proteins that are organized into six different subtypes: Type I/II keratin proteins expressed in epithelia cells, type III vimentin/desmin, type IV neurofilament proteins, type V nuclear lamins, and type VI nestin proteins expressed primarily in embryonic cells. Nestin has a conserved core region (amino acids 7 to 314), which contains an α helical domain that is involved in coiled-coil assembly of IFs. The C-terminal region of nestin is similar to type IV IFs, since it contains highly charged amino acids, many glutamate residues, and an 11 amino acid repeat motif. Nestin is expressed in the cerebrum during embryonic development, in the cerebellum during early postnatal development, and in dermatomal cells and myoblasts during myogenesis. In vitro, nestin forms homodimers and homotetramers, but not IFs, and can co-assemble with type III vimentin and type IV internexin proteins. Thus, nestin is a core IF protein that is essential for proper cytoskeletal formation during neurogenesis and myogenesis. Nestin molecular weight can vary anywhere from 200 to 260kDa depending on where it is being expressed.

Host Species: Mouse
Clone: 25
Isotype: IgG1, κ
Species Reactivity [for Features Main]: Rat
Immunogen: Rat Nestin aa. 402-604

Immunofluorescence, Western Blotting

Specifications

Specifications

Nestin
25/NESTIN
Unconjugated
Affinity Purified
Rat Nestin aa. 402-604 Recombinant Protein
50μg
Cell Biology
Primary
Human, Rat
Western Blot
250μg/mL
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Murine
IgG1, κ
RUO
Store undiluted at -20°C.
Monoclonal
Documents
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