Invitrogen™ NEFM Monoclonal Antibody (NF-09)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA119225

Catalog No. 01-675-989

Description

This antibody reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa intracellular antigen) of various species. Immunocytochemistry: Carnoys fixative 2 x 3 min, blocking 1% glycine + 0.2% gelatin 10 min.

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Specifications

• Antigen: NEFM
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: P07197, P08552, P08553, P12839
• Gene Symbols: NEFM
• Immunogen: Pellet of pig brain cold stable proteins after depolymerization of microtubules.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Pig, Mammalia
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: NF-09
• Conjugate: Unconjugated
• Gene: NEFM
• Gene Alias: 160 kDa neurofilament protein; major neuronal intermediate filament subunit; medium neurofilament subunit; middle molecular weight neurofilament protein NF-M(2); Nef3; Nefm; nef-m; nefm.S; nefm-a; nefm-b; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium; neurofilament medium polypeptide; neurofilament medium S homeolog; neurofilament medium tail domain; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neurofilament-M; neurofilament-M subunit; neuronal intermediate filament; NF160; NF165; NFM; NF-M; NF-M c-terminus; NF-M protein; NMC; XELAEV_18020229mg
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100625407, 18040, 24588, 4741
• Content And Storage: 4°C, do not freeze
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov