MilliporeSigma™ anti-Neurofilament H (200kDa), C-terminus ; Polyclonal; ™ (Chemicon™)

Supplier:  MilliporeSigma™ AB1989/DEL

Catalog No. 50-173-345

Description

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kDa NF-L protein can self-assemble into a filamentous structure, however the 160kDa NF-M and 200kDa NF-H proteins require the presence of the 68/70kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.

Immunohistochemistry, Western Blotting

Specifications

• Antigen: Neurofilament H (200kDa), C-terminus
• Conjugate: Unconjugated
• Immunogen: Recombinant fusion protein containing the extreme C-terminus of rat NF-H, prepared from E. coli inclusion bodies by dissolution in urea followed by DEAE-cellulose ion exchange chromatography. Immunogen does not include the lysine-serine-proline
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Serum

• Classification: Polyclonal
• Host Species: Rabbit
• Quantity: 50 μL
• Research Discipline: Neuroscience
• Target Species: Bovine, Human, Mouse, Pig, Rat