NFkB p65 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1186
PA1-186 has been successfully used in WB using StartingBlock T20 (TBS) Blocking Buffer (Product No. 37543). More non-specific bands are observed when using 5% BSA for blocking. By WB, PA1-186 detects a predomit band at 65kD. Loading increasing amounts of cell lysate may yield more intense nonspecific bands at ~120kD and/or ~50kD. PA1-186 has been succesfully used for IP of the NF-kB p65 subunit and co-IP of the NF-kB p50 subunit.NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases, and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.
|Antigen affinity chromatography|
|ChIP assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot|
|PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide|
|RelA; NFKB3; Nuclear Factor kB p65 subunit; p65; Transcription Factor p65; nf?b|
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