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NFkB p65 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1186

Catalog No. PIPA1186


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Description

Description

PA1-186 has been successfully used in WB using StartingBlock T20 (TBS) Blocking Buffer (Product No. 37543). More non-specific bands are observed when using 5% BSA for blocking. By WB, PA1-186 detects a predomit band at 65kD. Loading increasing amounts of cell lysate may yield more intense nonspecific bands at ~120kD and/or ~50kD. PA1-186 has been succesfully used for IP of the NF-kB p65 subunit and co-IP of the NF-kB p50 subunit.

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases, and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.
Specifications

Specifications

NFkB p65
Polyclonal
Unconjugated
RELA
Liquid
RELA
GLLSGDEDFSSIADMDFS
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Mouse
ChIP assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
1 mg/mL
PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
Q04206, Q04207
RelA; NFKB3; Nuclear Factor kB p65 subunit; p65; Transcription Factor p65; nf?b
Rabbit
IgG
100 μg
-20°C
Primary
19697, 5970
Documents
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