MilliporeSigma™ anti-Nibrin/Nbs1 Clone: EE15,

Mouse Monoclonal Antibody

Manufacturer:  MilliporeSigma™ 05616C

Catalog No. 05-616-CMI

Description

Specifically detects Nibrin/Nbs1 Clone: EE15 in Human samples, and it is validated for Immunocytochemistry, Immunoprecipitation, Western Blotting

Nibrin (UniProt O60934; also known as Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, p95 protein of the MRE11/RAD50 complex) is encoded by the NBN (also known as AT-V1, AT-V2, ATV, NBS, NBS1, P95) gene (Gene ID 4683) in human. Nibrin is a component of the MRN (MRE11/RAD50/NBN) complex involved in early steps of DNA double strand breaks (DSBs). The phosphorylation of H2AX pSer139 (γ-H2AX) found at the DNA-damaged sites, for example, is mediated by a direct interaction between Nibrin and the phosphorylated mediator of the DNA damage checkpoint 1 (MDC1). ATM, CtIP/RBBP8, Tip60, BRCA1, and SMC1 are also known interaction partners of Nibrin. Nibrin contains a fork-head associated (FHA) domain and two BRCA1 C-terminal (BRCT) tandem domains in its N-terminal region, followed by two MRE11-binding motifs and an ATM-binding motif in its C-terminal region. The Ser278 and Ser343 residues are known to be targeted for phosphorylation by ATM in response to ionizing-radiation (IR). NBN gene mutations are responsible for Nijmegen breakage syndrome (NBS), a disorder characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer.

Specifications

• Antigen: Nibrin/Nbs1
• Clone: EE15
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Format: Purified
• Host Species: Murine
• Isotype: IgG1, κ
• Quantity: 200μg
• Research Discipline: Epigenetics, Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_002476
• Target Species: Human

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: O60934
• Gene Symbols: NBN, NBS, NBS1, P95
• Immunogen: GST fusion protein corresponding to residues 420-774 of human Nibrin/Nbs1.
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C for one year from date of shipment
• Monoclonal or Polyclonal: Monoclonal
• Test Specificity: Recognizes Nibrin/Nbs1, Mr 95kDa. A non-specific protein was also detected at Mr 33kDa in some lysates, but not Jurkat cell lysate.

For Research Use Only