MilliporeSigma™ anti-Nitrotyrosine Rabbit Monoclonal,

Rabbit Monoclonal Antibody

Supplier:  MilliporeSigma™ 06284AF488

Catalog No. 06-284-AF488

Description

Specifically detects Nitrotyrosine Clone: in Human samples, and it is validated for Immunocytochemistry

Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species. Proteins are often targets of reactive species and detection of oxidatively modified proteins is often used as an index of oxidative stress. Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide. The reaction of proteins with peroxynitrite results in the oxidation of tryptophan and cysteine, as well as the nitration of tyrosine, formation of dityrosine, 2,4 dinitrophenylhydrazine-reactive carbonyls, and protein fragmentation. The formation of 3-nitrotyrosine represents a specific peroxynitrite-mediated protein modification that is different from modifications mediated by reactive oxygen species. Studies show that elevated nitrotyrosine levels are associated with inflammatory conditions, such as atherosclerotic plaques and rheumatoid arthritis.

Specifications

• Antigen: Nitrotyrosine
• Classification: Monoclonal
• Formulation: Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
• Immunogen: Nitrated KLH.
• Quantity: 100 μL
• Research Discipline: Signaling
• Test Specificity: Recognizes proteins which are nitrated.
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG

• Applications: Immunocytochemistry
• Conjugate: Alexa Fluor 488
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only