MilliporeSigma™ anti-Nitrotyrosine Rabbit Monoclonal,

Catalog No.	Quantity	Conjugate
06-284-AF488	100 μL	Alexa Fluor 488

Catalog No. 06-284-AF488 Supplier MilliporeSigma™ Supplier No. 06284AF488

Description

Rabbit Monoclonal Antibody

Specifically detects Nitrotyrosine Clone: in Human samples, and it is validated for Immunocytochemistry

Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species. Proteins are often targets of reactive species and detection of oxidatively modified proteins is often used as an index of oxidative stress. Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide. The reaction of proteins with peroxynitrite results in the oxidation of tryptophan and cysteine, as well as the nitration of tyrosine, formation of dityrosine, 2,4 dinitrophenylhydrazine-reactive carbonyls, and protein fragmentation. The formation of 3-nitrotyrosine represents a specific peroxynitrite-mediated protein modification that is different from modifications mediated by reactive oxygen species. Studies show that elevated nitrotyrosine levels are associated with inflammatory conditions, such as atherosclerotic plaques and rheumatoid arthritis.

Specifications

• Antigen: Nitrotyrosine
• Applications: Immunocytochemistry
• Classification: Monoclonal
• Conjugate: Alexa Fluor 488
• Formulation: Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
• Host Species: Rabbit
• Immunogen: Nitrated KLH.
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Test Specificity: Recognizes proteins which are nitrated.
• Target Species: Human
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Form: Purified
• Isotype: IgG

For Research Use Only