NK-T/NK Cell Antigen (ICAM-1) Rat anti-Mouse, PE, Clone: U5A2-13, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 550082

Catalog No. BDB550082

Description

The U5A2-13 mAb recognizes an antigen expressed on most-NKT cells and 55% of NK cells in the liver of various mouse strains, including both NK1.1+ (e.g., C57BL/6) and NK1.1- (e.g., BALB/c, C3H/He, MRL/lpr) strains. Abundant IL-4 and IFN-γ are produced by U5A2-13 Ag+ TCR αβ+ cells upon CD3 cross-linking, but not by U5A2-13 Ag- TCR αβ+ cells in both NK1.1+ and NK1.1- strains. In C57BL/6 mice (NK1.1+ strain), 80%of U5A2-13 Ag+ T cells co-express the NK1.1 molecule. When staining hepatic mononuclear cells with U5A2-13 mAb, the fluorescence intensity detected on on CD3- cells is lower than on CD3+ cells in BALB/c and C57BL/6 mice. Immunoprecipitation studies have shown that the U5A2-13 antigen comprises three proteins of 65, 34, and 31kDa. This antibody clone U5A2-13 was originally established for mouse NKT cells, but recent studies showed that it recognized a novel conformational epitope of ICAM-1.

Host Species: Rat
Clone: U5A2-13
Isotype: IgG2a κ
Species Reactivity: Mouse
Immunogen: BALB/c nu/nu mouse lymphoma line tMK-2U

Flow Cytometry

Specifications

• Antigen: NK-T/NK Cell Antigen (ICAM-1)
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: ICAM-1
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: U5A2-13
• Conjugate: PE
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: BALB/c nu/nu mouse lymphoma line tMK-2U
• Quantity: 0.2 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2a κ

For Research Use Only.