Nkx2.2 Mouse, PE, Clone: 74.5A5, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 564730

Catalog No. BDB564730

Description

The 74.5A5 monoclonal antibody specifically binds to Homeobox protein Nkx-2.2 (Nkx2.2), which is a transcription factor required for the differentiation of ventral neuronal populations in the central nervous system and endocrine cell populations in the pancreas and intestine. Within the pancreas, Nkx2.2 is expressed in many cell subsets and plays important roles in islet and β- cell differentiation and maturation. Mice with a homozygous disruption of the Nkx2-2 gene lack mature β cells and die shortly after birth with severe hyperglycemia. Similarly to the Nkx2.2-ablated mice, homozygous mutations in the human NKX2-2 gene have been described in diabetic patients and associated with the etiology of neonatal diabetes. The NKX2-2 gene is also a target of the Ewing sarcoma specific protein EWS-FLI. Nkx2.2 expression can be monitored in order to determine cell fate changes in cultures for the generation of human pluripotent stem cell-derived insulin-producing cells as well as oligodendrocyte progenitors.

Specifications

• Antigen: Nkx2.2
• Clone: 74.5A5
• Description: NKX2-2; NKX2B; Nkx2-2; Nkx-2.2; NK-2 homolog B; glycoprotein GP330
• Format: Affinity Purified
• Immunogen: Chicken Nkx2.2 Recombinant Protein
• Quantity: 50 Tests
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry
• Conjugate: PE
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Murine
• Isotype: IgG_2b
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chicken, Human, Murine, Rat

For Research Use Only.