NPM-ALK Set Mouse anti-Human, PE, Clone: ALK1, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 559257

Catalog No. BDB559257

Description

Reacts with ALK-NPM, a chimeric gene product of approximately 80 kDa, which is the result of t(2;5)(p23;q35) chromosomal translocation. The gene at 5q35 encodes for the cell cycle-regulated nucleolar protein, nucleophism (NPM), while the gene at 2p23 encodes a novel receptor tyrosine kinase, anaplastic lymphoma kinase (ALK). The 2;5 translocation results in the expression of NPM-ALK protein, or p80, comprised of the N-terminal portion of NPM (about 40% of the molecule) linked to the entire intracellular portion of ALK. ALK1 antibody recognizes a formalin resistant epitope in both the NPM-ALK chimeric and the 200 kDa normal human ALK proteins. Cytoplasmic and nuclear expression of NPM-ALK is seen in t(2;5)+ cell lines Su-DHL-1 and Karpas 299.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: NPM-ALK Set
• Classification: Monoclonal
• Conjugate: PE
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Includes: PE Mouse Anti-Human NPM-ALK/ALK (100 tests ), PE Mouse IgG3 Kappa Isotype Control (anti-Fructosan) (100 tests)
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: ALK1
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG3 κ