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NSF Mouse, Unlabeled, Clone: 7, BD

Catalog No. BDB612273 Shop All BD Biosciences Products
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BDB612273 150 μg
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Catalog No. BDB612273 Supplier BD Biosciences Supplier No. 612273

Mouse Monoclonal Antibody

Eukaryotic protein trafficking involves the packaging of target molecules into membraneous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Components of both the vesicle and the plasma membrane interact to form a fusion complex, which mediates specific docking and fusion of vesicles. This complex contains NSF (N-ethyl-maleimide-sensitive factor), SNAPs (soluble NSF attachment proteins), and receptor proteins (SNAREs). SNAP-25 (synaptosome-associated protein of 25kDa) and syntaxin are SNAREs that are associated with the target plasma membrane (t-SNAREs), while synaptobrevin and synaptotagmin are vesicle-associated SNARE proteins (v-SNAREs). SNAREs form a 7S complex that has a high affinity binding site for α-SNAP and NSF. Upon binding of α-SNAP and NSF, a 20S complex is formed that is rapidly disassembled due to NSF's ATPase activity. This facilitates vesicle fusion to the target membrane followed by fusion and release of vesicle components.

Immunofluorescence, Western Blotting

TRUSTED_SUSTAINABILITY

Specifications

Antigen NSF
Applications In vitro Assay, Immunofluorescence, Western Blot
Classification Monoclonal
Clone 7
Concentration 250μg/mL
Conjugate Unconjugated
Host Species Mouse
Immunogen Human NSF aa. 488-601
Purification Method Affinity Purified
Quantity 150 μg
Regulatory Status RUO
Research Discipline Cell Biology
Primary or Secondary Primary
Target Species Canine, Chicken, Human, Mouse, Rat
Isotype IgG2b
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For Research Use Only.

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