Oct-2, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA138329
Oct-2 is a transcription factor belonging to the POU homeo-domain family that binds to the Ig gene octomer sites regulating B cell specific genes. Oct-2 protein expression is not restricted to B cells, although expression levels are much higher in these cells. Oct-2 expression is reported to be significantly greater in germinal center derived lymphomas, although other B cell lymphomas also display high levels of expression.This antibody is predicted to react with canine, mouse, porcine, rabbit based on sequence homology.Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
|Synthetic peptide derived from C-terminus of human Oct-2|
|Antigen affinity chromatography|
|Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|PBS with 1% BSA and <0.1% sodium azide; pH 7.6|
|OCT2, OTF2, Oct-2|
|4° C, do not freeze, do not freeze|
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