p120 Catenin (pT310) Mouse anti-Human, PE, Clone: 22/P120 (PT310), BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 558596

Catalog No. BDB558596

Description

The membrane-associated protein pp120 Src substrate (p120 catenin, p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src-transformed cells. p120 catenin is localized to the E-Cadherin/catenin cell adhesion complex. Like β- and γ-catenin, p120 catenin directly associates with the cytoplasmic C terminus of E-Cadherin via its Arm domain. It exists as four isoforms that range in size from 90 to 115kDa. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered expression contributes to malignancy. Phosphorylation of multiple serine (S252, S268, S288, and S873) and threonine (T310 and T910) residues in p120 catenin may regulate its activity. The S873 residue is phosphorylated after PKC activation, while the S268 site is dephosphorylated after PKC activation. The latter residue is phosphorylated in vitro by p160 Rock. S252 and T310 residues are phosphorylated in vitro by GSK3b. Thus, p120 catenin function may be regulated in a complex manner through both serine and threonine phosphorylation. The 22/p120 (pT310) monoclonal antibody recognizes the phosphorylated T310 in the regulatory domain of p120 catenin.

Intracellular Staining

Specifications

• Antigen: p120 Catenin (pT310)
• Classification: Monoclonal
• Conjugate: PE
• Immunogen: Phosphorylated Human p120 Catenin Peptide
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: 22/P120 (PT310)
• Host Species: Mouse
• Quantity: 50 Tests
• Research Discipline: Cell Biology
• Target Species: Human