MilliporeSigma™ anti-p14ARF (Ab-1) (118-132), Polyclonal

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ PC409100UL

Catalog No. PC409100UL

Description

Specifically detects p14^ARF (118-132) Clone: in Human samples, and it is validated for Immuno Blotting

The human p14ARF protein (murine homologue is p19ARF) results from the translation of an alternative reading frame of the CDKN2A (p16INK4) gene. Theα transcript gives rise to the p16 gene product while theβ transcript gives rise to the p14ARF gene product. Although transcriptionally derived from shared exons, there is little amino acid sequence homology between the two proteins. The function of the p14ARF protein is to activate a p53 response and thereby induce cell cycle arrest. p53 in turn negatively regulates p14ARF expression. The mechanism of action for p14ARF is a direct binding of the COOH terminal domain of MDM2 which in turn forms a ternary complex with p53. Interaction with MDM2 results in the blocking of the MDM2 induced degradation of p53. p14ARF transcription is also modulated by the activity of E2F1 expression. Loss of p14ARF correlates with loss of p16 expression and alteration in expression of both proteins is frequently seen in non-small cell lung carcinoma.

Specifications

• Antigen: p14^ARF (118-132)
• Classification: Polyclonal
• Formulation: Undiluted serum.
• Immunogen: a synthetic peptide [(K)AGRARCLGPSARGPG] corresponding to amino acids 118-132 of human p14^ARF (p16β)
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽14kDa p14^ARF protein in Saos-2 cells. Sold under license of U.S. Patent 6,482,929.
• Content And Storage: −20°C
• Isotype: IgG

• Applications: Immunoblot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only