MilliporeSigma™ anti-p21WAF1 Clone: DF10,

Catalog No.	Quantity	Conjugate
OP68100UG	100 μg	Unconjugated

Catalog No. OP68100UG Supplier MilliporeSigma™ Supplier No. OP68100UG

Description

Mouse Monoclonal Antibody

Specifically detects p21^WAF1 Clone: DF10 in Human samples, and it is validated for Immunoprecipitation

The tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ∽21kDa product of the WAF1 gene is found in a complex involving cyclins, cyclin dependent kinases (CDK), and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is to prevent CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. However, the role of p21WAF1 in apoptosis is less clear although p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 in response to DNA damage can occur by both p53-dependent and p53-independent mechanisms, in response to mitogenic stimuli, differentiation, or in tumor cells with mutated p53. Functional p21WAF1 is essential for p53-mediated G1 arrest presumably due to WAF1 inhibition of both CDK activity and PCNA-dependent DNA replication. WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Not surprisingly, p21WAF1 overexpression is growth suppressive, consistent with its role as an inhibitor of CDKs. By inhibiting Rb inactivation in a p53-dependent fashion, p21WAF1 serves to integrate cell cycle control mediated by p53 and Rb.

Specifications

• Antigen: p21^WAF1
• Applications: Immunoprecipitation
• Classification: Monoclonal
• Clone: DF10
• Conjugate: Unconjugated
• Formulation: In 0.05M sodium phosphate buffer, 0.2% gelatin.
• Host Species: Mouse
• Immunogen: full-length, recombinant human p21^WAF1
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽21kDa p21^WAF1 protein in skin or colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
• Target Species: Human
• Content And Storage: 2°C to 8°C
• Form: Purified
• Isotype: IgG1

For Research Use Only