MilliporeSigma™ anti-p300 Clone: NM-11,

Catalog No.	Quantity	Conjugate
NA46100UG	100 μg	Unconjugated

Catalog No. NA46100UG Supplier MilliporeSigma™ Supplier No. NA46100UG

Description

Mouse Monoclonal Antibody

Specifically detects p300 Clone: NM-11 in Human, Rat samples, and it is validated for Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Immuno Blotting

p300 is a nuclear phosphoprotein which was originally shown to specifically bind to adenovirus E1A protein. The binding of p300 to E1A occurs in the conserved region 1 (CR1) of E1A at a site which is close to but non-overlapping with that for the Rb family of proteins including p105 and p107. p300 has been shown to bind to the extreme N-terminal region requiring the presence of positively charged residues at positions 2 and 3 of E1A via a zinc-finger motif in the C-terminal portion of p300. While the binding of p300 and Rb to E1A is independent, it has been shown that upon binding, E1A mediates the physical contact between the two proteins. The p300 protein is actively phosphorylated in both quiescent and proliferating cells however the amount of phosphorylation increases during progression from late G1 intoM phase of the cell cycle. In addition to the demonstrated binding of p300 to E1A it has also been shown to bind to other molecules including DNA (in a site specific manner) and to components of the TATA binding protein complexes (TBP). The TBP complex association is most likely with TFIID and occurs in a protein-protein dependent rather than a DNA dependent manner and, in such a complex, p300 functions as a co-activator of transcription.

Specifications

• Antigen: p300
• Applications: Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
• Classification: Monoclonal
• Clone: NM-11
• Conjugate: Unconjugated
• Formulation: In 0.05M sodium phosphate buffer, 50% glycerol.
• Host Species: Mouse
• Immunogen: recombinant, human p300 protein
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the p300 protein in A431, HeLa, and ML-1 cells.
• Target Species: Human, Rat
• Content And Storage: −20°C
• Form: Purified
• Isotype: IgG1

For Research Use Only