p38α Mouse, Unlabeled, Clone: 27, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 612168
Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytic cells results in the production and release of proinflammatory cytokines, such as IL-1 and TNF-α. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the mitogen activated protein kinases (MAPKs). One of these, p38 MAP kinase (p38 MAPK or CSPB2), is a human homologue of HOG1, a yeast MAP kinase that is essential for growth under conditions of elevated osmolarity. Similar to HOG1, p38 MAPK is phosphorylated in response to hyperosmolarity, but also in response to a variety of stimuli including LPS, IL-1, or TNF-α. Efficient activation of p38 MAPK requires phosphorylation of Thr-180 and Tyr-182. At least three Thr/Tyr kinases (MKK3, MKK4/SEK1, and MKK6) phosphorylate and activate p38 MAPK. This leads to the activation of multiple transcription factors (NF-κB, ATF-2, Elk-1, and CHOP/GADD143) that induce expression of proinflammatory cytokine genes. In addition to its role in immunity, p38 MAPK is widely expressed and serves as an important signal transducer in a variety of cell types.
Immunofluorescence, Western Blotting
|Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.|
|Store undiluted at -20°C.|
|Human p38 MAPK aa. 243-355|
|Canine, Human, Murine, Rat|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok
For Research Use Only.