MilliporeSigma™ anti-p53 (Pantropic) Clone: PAb1801,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ OP09100UG

Catalog No. OP09100UG

Description

Specifically detects p53 (Pantropic) Clone: PAb1801 in Human samples, and it is validated for Gel Supershift, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Immuno Blotting

The human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.

Specifications

• Antigen: p53 (Pantropic)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

• Applications: Gel Supershift, Immunoblot, Immunoblot, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
• Clone: PAb1801
• Formulation: In 50mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
• Immunogen: a human p53 fusion protein
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽53kDa wild-type and mutant p53 protein in A-431 cells and breast carcinoma tissue.
• Content And Storage: 2°C to 8°C
• Isotype: IgG1

For Research Use Only