p53 Mouse, FITC, Clone: DO-7, BD

Catalog No.	Quantity
BDB554298	100 Tests

Catalog No. BDB554298 Supplier BD Biosciences Supplier No. 554298

Description

Includes

FITC Mouse Anti-Human p53, FITC Mouse IgG2b Isotype Control (anti-dansyl)

Mouse Monoclonal Antibody

p53 is a 53kDa nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wild-type p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. DO-7 recognizes human wildtype and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-7 recognizes an epitope between amino acids 1-45 of the known forms of human p53. Human recombinant p53 protein was used as immunogen. Wildtype 53 proteins have a very short half-life and are usually not detectable with monoclonal antibodies in normal tissues. Clone DO-7 was initially characterized by immunoprecipitation, western blot analysis, immunohistochemistry of frozen and formalin-fixed paraffin-embedded tissue sections, and flow cytometric analysis.
Clone 27-35 is an isotype control monoclonal antibody specific for hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl). This hapten is not expressed on human cells or human cell lines. The DO-7 and 27-35 FITC conjugates are matched in F/P ratios. The optimum F/P ratio was experimentally determined by flow cytometric analysis.

Host Species: Mouse
Clone: DO-7, 27-35
Isotype: IgG2b, IgG2b, κ
Species Reactivity: Human

Flow Cytometry

Specifications

• Antigen: p53
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: DO-7
• Conjugate: FITC
• Host Species: Mouse
• Purification Method: Affinity Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Bovine, Human, Monkey
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b

For Research Use Only.