p54nrb Mouse, Unlabeled, Clone: 3, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 611279

Catalog No. BDB611279

Description

Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes that contain multiple snRNPs and a number of non-snRNP splicing factors, such as hPRP17 and hPRP18. p54 [nrb] (nuclear RNA-binding protein, 54 kDa) is a non-snRNP RNA splicing factor. It binds and enhances the activity of topoisomerase I, the enzyme that nicks DNA to relieve torsional stress during replication and transcription. p54 [nrb] is the the human homolog of mouse NonO and bovine IPEB. The N-terminal half of p54 [nrb] contains two RNA recognition motifs (RRMs), that allow it to bind single stranded RNA. These motifs are included in a region that is 71% homologous with the human splicing factor PSF and 42% homologous with the Drosophila puff-specific protein BJ6. In addition to its role in RNA splicing, p54 [nrb] functions as a DNA transcription factor in multiple cell types. The N-terminal portion of p54 [nrb] also mediates its interaction with DNA. The ability of p54 [nrb] to bind DNA, but not RNA, is thought to require post-translational modification. Thus, p54 [nrb] can function as either an RNA splicing factor or a DNA transcription factor in a post-translational-dependent manner.

Specifications

• Antigen: p54nrb
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Human p54 [nrb] aa. 368-471
• Quantity: 150 μg
• Research Discipline: Cell Biology
• Target Species: Canine, Human, Mouse, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 3
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.