MilliporeSigma™ anti-p73 Ab 2, Clone: ER-15,

Catalog No.	Quantity	Conjugate
OP109100UG	100 μg	Unconjugated

Catalog No. OP109100UG Supplier MilliporeSigma™ Supplier No. OP109100UG

Description

Mouse Monoclonal Antibody

Specifically detects p73 Clone: ER-15 in Human, Monkey samples, and it is validated for Gel Supershift, Immunoprecipitation, Immuno Blotting

The p73 protein shares considerable structural and functional similarity to p53. The p73 gene encodes 4 polypeptides through alternative splicing of exons 11-13 with p73α and p73β expressed in all tissues while expression of p73γ and p73δ may be more restricted. All isoforms show significant sequence identity to the transactivation, DNA binding, and oligomerization domains of p53, and many of the critical amino acid residues in p53 essential for DNA binding are conserved in p73. Not surprisingly, p73 binds to a p53 consensus sequence and transactivates many, but not all, p53-responsive genes. Overproduction of p73 leads to growth arrest and induction of apoptosis in a p53-independent manner. However, p73 does not transactivate all p53-responsive genes and the level of transactivation is often lower than that by p53; thus, the mechanisms of growth arrest and induction of apoptosis by p73 are at least partially distinct from p53. p73 does not appear to be targeted by many of the viral oncoproteins such as E1B 55K, SV40 T antigen, and HPV E6 that bind to and inactivate p53, however both p53 and p73 do bind the Adenovirus E4orf6 protein, suggesting that p73 may be independently inactivated by viral oncoproteins. All p73 isoforms bind one another and p73α and p73β appear to bind p53 as well. Interestingly, mutant p53 binds p73α and inhibits transcriptional activation by p73 indicating that mutational inactivation of p53 may serve to simultaneously inactivate p73. One key difference between p53 and p73 is that, unlike p53, p73 does not appear to be activated in response to DNA damage.

Specifications

• Antigen: p73
• Applications: Gel Supershift, Immunoblot, Immunoblot, Immunoprecipitation
• Classification: Monoclonal
• Clone: ER-15
• Conjugate: Unconjugated
• Formulation: In 0.05M sodium phosphate buffer, 0.2% gelatin.
• Host Species: Mouse
• Immunogen: recombinant, human p73α protein
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽80kDa α and the ∽68kDa β isoforms of p73 in HEK293, LOS, and IM9 cells. Will immunoprecipitate p73α/p73β complexes.
• Target Species: Human, Monkey
• Content And Storage: 2°C to 8°C
• Form: Purified
• Isotype: IgG1

For Research Use Only