MilliporeSigma™ anti-Pan-Ras Clone: RAS 10,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ OP40100UG

Catalog No. OP40100UG

Description

Specifically detects Pan-Ras Clone: RAS 10 in Human, Mouse, Rat samples, and it is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Immuno Blotting

The human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.

Specifications

• Antigen: Pan-Ras
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Flow Cytometry, Immunoblot, Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
• Clone: RAS 10
• Formulation: In 50mM sodium phosphate buffer, 0.2% gelatin.
• Immunogen: recombinant human p21 Ras
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽21kDa H-, K-, and N-Ras proteins in SW480 and Y1 cells and breast carcinoma tissue.
• Content And Storage: 2°C to 8°C
• Isotype: IgG2a

For Research Use Only