MilliporeSigma™ anti-PAR Clone: 10H,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ AM80100UG

Catalog No. AM80100UG

Description

Specifically detects PAR Clone: 10H in Drosophila, Human, Mouse, Rat samples, and it is validated for Immunocytochemistry

This monoclonal antibody is directed against poly(ADP)-ribose (PAR) which is synthesized by the nuclear DNA repair enzyme poly(ADP)-ribose polymerase (PARP). PARP is selectively activated when DNA strands break and catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, such as PARP. The amount of PAR formed in living cells with DNA damage is directly related to the extent of the damage. When DNA is damaged, PAR undergoes a rapid turnover, with a half-life only minutes long, as it is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP)-ribose glycohydrolase (PARG). Upon DNA damage by γ-irradiation or oxidative stress PAR fluorescence can be detected for approximately 10 min. PARP is strongly activated during the so-called execution phase of apoptosis, causing a second round of PAR synthesis. PAR synthesized early in apoptosis is remarkably stable and its immunofluorescence correlates well with other markers of apoptosis.

Specifications

• Antigen: PAR
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Drosophila, Human, Mouse, Rat
• Form: Purified

• Applications: Immunocytochemistry
• Clone: 10H
• Formulation: In 100mM NaCl, 50mM HEPES, 1% BSA, pH 7.4.
• Immunogen: purified poly(ADP-ribose)
• Regulatory Status: RUO
• Test Specificity: Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.
• Content And Storage: −20°C
• Isotype: IgG3

For Research Use Only