PARP Mouse anti-Human, Clone: 194C1439, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen MA141020
Suggested positive control: camptothecin treated HL-60 (12 hr).The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.
|PBS with 0.05% BSA and 0.05% sodium azide|
|Cleavage specific PARP|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Flow Cytometry, Western Blot|
|Synthetic peptide corresponding to residues near the 214/215-cleavage site of human PARP.|
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