PARP Mouse anti-Human, Unlabeled, Clone: 4C10-5, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 556494

Catalog No. BDB556494

Description

PARP [Poly(ADP-ribose) polymerase] is a 113kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in non-apoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA damage. Additionally, PARP is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from a 113kDa intact form into smaller 89kDa and 24kDa fragments. This process separates the amino-terminal DNA-binding domain of the enzyme from the carboxy-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis. The 4C10-5 antibody recognizes both the intact 113kDa form and 89kDa fragment of PARP. The 4C10-5 antibody has been reported to recognize both native and denatured PARP. Purified human PARP was used as the immunogen and the antibody reported to react with an epitope located in the NAD binding domain. In dot blot assays, the antibody reacts with the native enzyme in the presence or absence of bound DNA as well as after synthesis of covalently linked poly (ADP-ribose). The 4C10-5 antibody is routinely tested by western blot analysis of untreated Jurkat T cells and Jurkat T cells induced to undergo apoptosis. This antibody is routinely tested by Western blot analysis and immunofluorescent imaging. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Mouse
Clone: 4C10-5
Isotype: IgG1 κ
Species Reactivity: Human
Immunogen: Human PARP

Bioimaging, Dot Blotting, Flow Cytometry, Immunoprecipitation, Intracellular Staining, Western Blotting

Specifications

• Antigen: PARP
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Human PARP
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Western Blot
• Clone: 4C10-5
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.