PARP Mouse anti-Human, Unlabeled, Clone: 7D3-6, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 551024

Catalog No. BDB551024

Includes: Purified Mouse Anti-Human PARP, Jurkat Cell Lysate

Description

PARP [Poly(ADP-ribose) polymerase] is a 116kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in nonapoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA damage. Additionally, PARP is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from the 116kDa intact form into 85kDa and 25kDa fragments. This process separates the amino-terminal DNA-binding domain of the enzyme from the carboxy-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis.

Host Species: Mouse
Clone: 7D3-6
Isotype: IgG1
Species Reactivity: Human
Immunogen: Purified human PARP

Flow Cytometry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: PARP
• Classification: Monoclonal
• Concentration: 0.25mg/mL
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

• Applications: Western Blot
• Clone: 7D3-6
• Conjugate: Unconjugated
• Immunogen: Purified human PARP
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1

For Research Use Only.