Paxillin Mouse, Unlabeled, Clone: 349, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 612405

Catalog No. BDB612405

Description

A number of cytoskeletal proteins are tyrosine phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts. One of these is the 68kDa paxillin protein. Paxillin is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers. It is also present in the focal adhesions of Madin-Darby canine kidney epithelial cells, but is absent from the cell adherens junctions of these cells. Paxillin purified from chicken gizzard migrates as a diffuse band on SDS-PAGE with molecular weight of 65-70kDa. It binds to the rod domain of vinculin, another focal adhesion protein. It is thought that phosphorylation of paxillin may have a role in that disassembly of focal adhesions and stress fibers during transformation.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: Paxillin
• Classification: Monoclonal
• Concentration: 1.0mg/mL
• Formulation: Aqueous buffered solution containing glycerol and ≤0.09% sodium azide.
• Immunogen: Chicken Paxillin aa. 1-557
• Quantity: 100 μg
• Research Discipline: Cell Biology
• Target Species: Canine, Chicken, Human, Mouse, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 349
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov