Perforin Set anti-Human, FITC, Clone: +BD7238, BD

Monoclonal Antibody

Supplier:  BD Biosciences 556577

Catalog No. BDB556577

Description

Perforin has a key role in cell-mediated cytotoxicity. It is a 70 kDa cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses, in allograft rejections, and in some autoimmune diseases. NK cells are important for tumor surveillance and destruction and are involved in allograft rejections. Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell. In the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that resembles apoptosis. The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding perforin-mediated reactions.

Clone δ G9 reacts with human and bovine perforin. It does not cross-react with mouse perforin. Purified granules from the human lymphoma cell line YT were used as immunogen. Clone δ G9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen tissue sections. The antibody stains scattered lymphocytes in red pulp of spleen, and scattered infiltrated lymphocytes in lymphoma.

Specifications

• Antigen: Perforin Set
• Classification: Monoclonal
• Conjugate: FITC
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG2b

• Applications: Flow Cytometry
• Clone: +BD7238
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Includes: FITC Mouse Anti-Human Perforin (100 tests), FITC Mouse IgG2b κ Isotype Control (100 tests)
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.