Perforin Mouse anti-Human, PE, Clone: ΔG9, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 556437

Catalog No. BDB556437

Includes: PE Mouse Anti-Human Perforin, PE Mouse IgG2b, κ Isotype Control (anti-dansyl)

Description

Perforin has a key role in cell-mediated cytotoxicity. It is a 70kDa cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses, in allograft rejections, and in some autoimmune diseases. Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell. In the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that resembles apoptosis. The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding perforin-mediated reactions. Clone δG9 reacts with human and bovine perforin. It does not cross-react with mouse perforin. Clone δG9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen tissue sections, and has also been published for flow cytometric analysis. Clone 27-35 is an isotype control monoclonal antibody specific for hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl). This hapten is not expressed on human cells or human cell lines.

Host Species: Mouse
Clone: δG9, 27-35
Isotype: IgG2b, κ
Species Reactivity: Human
Immunogen: Purified Granules from the Human Lymphoma Cell Line YT

Flow Cytometry

Specifications

• Antigen: Perforin
• Classification: Monoclonal
• Conjugate: PE
• Immunogen: Purified Granules from the Human Lymphoma Cell Line YT
• Quantity: 100 Tests
• Research Discipline: Immunology
• Target Species: Human
• Isotype: IgG2b

• Applications: Flow Cytometry
• Clone: ΔG9
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.