Invitrogen™ PRDX4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA3753

Catalog No. PA3753

Description

PA3-753 detects Peroxiredoxin 4 protein in human samples. This antibody is specific for Prx 4 and shows no cross-reactivity with other Prx isoforms. PA3-753 has successfully been used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~23 kDa and ~20 kDa protein representing pro-Prx 4 and mature Prx 4, respectively, in human PC3 cell lysate. The PA3-753 antigen is purified human recombinant Prx 4 from K562 cells.

Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30 kilodalton in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol.

Specifications

• Antigen: PRDX4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PRDX4
• Gene Alias: Antioxidant enzyme AOE372; AOE372; AOE37-2; EC 1.11.1.15; epididymis secretory sperm binding protein Li 97n; HEL-S-97n; peroxiredoxin 4; peroxiredoxin IV; Peroxiredoxin-4; Prdx4; Prx 4; Prx IV; Prx4; PRX-4; prx-IV; Rno2CysPrx04; Thioredoxin dependent peroxide reductase A0372; thioredoxin peroxidase (antioxidant enzyme); thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372; TRANK
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Concentration: Conc. Not Determined
• Formulation: whole serum with 0.05% sodium azide
• Gene Accession No.: Q13162
• Gene Symbols: PRDX4
• Immunogen: Purified human recombinant Peroxiredoxin 4.
• Regulatory Status: RUO
• Gene ID (Entrez): 10549
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid