PFKM, Rabbit, Monoclonal Antibody, Abnova™

Rabbit monoclonal antibody raised against a human PFKM peptide using ARM Technology.

Supplier:  Abnova Corporation H00005213K

Catalog No. 89-200-816

Includes: Up to three rabbit IgG clones of 1mg each will be delivered to customer.

Description

The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM

Library Construction: Nonfusion antibody library from rabbit spleen (ARM Technology)
Expression: Overexpression vector and transfection into 293H cell line

Order Info

1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.

Specifications

• Antigen: PFKM
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: In 1X PBS, pH 7.4
• Gene Accession No.: PFKM
• Gene Symbols: PFKM
• Immunogen: A synthetic peptide of human PFKM
• Regulatory Status: RUO
• Gene ID (Entrez): 5213
• Content And Storage: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
• Isotype: IgG

• Applications: ELISA, Western Blot
• Concentration: 1mg
• Description: phosphofructokinase, muscle
• Gene: PFKM
• Gene Alias: GSD7, MGC8699, PFK-1, PFK-M, PFKX
• Host Species: Rabbit
• Quantity: 1 mg x up to 3 Abs
• Primary or Secondary: Primary
• Target Species: Human
• Form: Liquid