Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Phospho-CDK1 (Tyr15) Rabbit anti-Human, Rat, Xenopus laevis, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA14617

Catalog No. PA14617


Add to cart

Description

Description

This antibody is predicted to react with mouse and zebrafish based on 100% sequence homology. This antibody is specific for the ~38 kDa cdc2 protein phosphorylated at Tyr15 in Western blots of SK-N-MC total cell extracts. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

CDK1 (CDC2, P34CDC2) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDK1 also play important regulatory roles in cell cycle control. Destruction of cyclin B during metaphase results in inactivation of CDK1, allowing mitotic exit and cell division. CDK1 also contributes to the control of DNA replication and can be inhibited by several transcriptional targets of p53, such as p21WAF. The ability of CDK1 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2. Research studies have shown that expression of CDK1 in the brain has been linked to both neurogenesis and apoptosis. In vertebrates, multiple cyclins like CDK1 have been identified, including a total of eight such regulatory proteins in mammals. Alternatively spliced transcript variants encoding different isoforms of CDK1 have been found.
Specifications

Specifications

Phospho-CDK1 (Tyr15)
Polyclonal
0.01M HEPES with 0.15M NaCl, 100μg/mL BSA, 50% glycerol and no preservative; pH 7.5
P06493, P39951, P35567
CDK1, Cell division cycle 2 protein, Cyclin Dependent Kinase 1, p34 Protein Kinase
Rabbit
IgG
100 μL
-20° C, Avoid Freeze/Thaw Cycles
Primary
380246, 54237, 983
Western Blot
Unconjugated
CDK1
Liquid
CDK1, cdk1.S
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 of rat CDK1 conjugated to KLH
Affinity chromatography
RUO
Antibody
Polyclonal
Human, Rat, Xenopus laevis
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit