MilliporeSigma™ anti-phospho-Filamin-A (Ser2152) Clone: PS2,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABN1834

Catalog No. MABN1834MI

Description

Specifically detects Filamin-A (Ser2152) Clone: PS2 in Human, Mouse samples, and it is validated for Immunocytochemistry, Immunofluorescence, Western Blotting

Filamin-A (UniProt P21333; also known as ABP-280, Actin binding protein 280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, FLN-A, Non-muscle filamin) is encoded by the FLNA (also known as ABP, CSBS, CVD1, FLN, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD) gene (Gene ID 2316) in human. Filamin-A (FLN-A) is an actin-binding protein essential for cytoskeletal rearrangement and cell locomotion. Serine 2152 of FLN-A is a PKA phosphorylation target. FLN-A S2152 phosphorylation is essential for FLN-A-mediated recruitment of signaling proteins, adhesion molecules, and the vesicle-trafficking complex to the peripheral actin rim, so as to stabilize the cortical structure and epithelial membrane. Mutations in FLNA and ARFGEF2 genes cause periventricular heterotopia (PVH or PH), a congenital malformation of cortical development characterized by a reduced brain size and ectopic neuronal nodules along the ventricular neuroepithelium as a result of impaired initiation of neuronal migration. Suppression of either Arfgef2 or Mekk4 results in increased cellular FLN-A level and Ser2152 phosphorylation. In addition, Arfgef2 or Mekk4 deletion causes PVH in knockout mice.

Specifications

• Antigen: Filamin-A (Ser2152)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: P21333
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Ser2152 of human and murine Filamin-A isoform 1 (P21333-1 and Q8BTM8-1) corresponds to Ser2144 of human Filamin-A isoform 2 (P21333-2). Equivalent site is not present in murine spliced isoform 2.
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG2b κ

• Applications: Immunocytochemistry, Immunofluorescence, Western Blot
• Clone: PS2
• Formulation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: FLNA, FLN, FLN1
• Immunogen: KLH-conjugated linear peptide corresponding to the Filamin repeat 20 of human phospho-Filamin-A (Ser2152).
• Quantity: 100 μL
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_001447
• Target Species: Human, Mouse
• Form: Purified

For Research Use Only