Phospho-p70 S6 Kinase (Thr421), Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA536835

Catalog No. PIPA536835

Description

p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal.Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region. Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression. Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase.

This antibody detects endogenous protein at a molecular weight of 59, 70 and 85 kDa. It also detects p85 S6 kinase when phosphorylated at Thr444.Purity is >;95% by SDS-PAGE.

Specifications

• Antigen: Phospho-p70 S6 Kinase (Thr421)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RPS6KB1
• Gene Alias: Ribosomal protein S6 kinase I, S6K, S6K1, 70kDa ribosomal protein S6 kinase 1, p70 S6 kinase alpha, p70 S6K, p70-S6K, P70S6K, p70 S6 kinase alpha, Name: RPS6KB1, STK14A, p80 S6K1, p85 S6 kinase, p85 S6K, p85S6K
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6198, 72508, 83840
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1mg/mL
• Formulation: PBS with 50% glycerol and 0.1% sodium azide
• Gene Accession No.: P23443, Q8BSK8, P67999
• Gene Symbols: RPS6KB1
• Immunogen: Synthetic phosphopeptide derived from human p70 S6K around the phosphorylation site of Threonine 421
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG