MilliporeSigma™ anti-PhosphoDetect c-Raf (pTyr340, Tyr341), Polyclonal

Catalog No.	Quantity	Conjugate
55-300-910T	10 Tests	Unconjugated

Catalog No. 55-300-910T Supplier MilliporeSigma™ Supplier No. 55300910T

Description

Rabbit Polyclonal Antibody

Specifically detects PhosphoDetect™ c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Clone: in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry

Theraf family of proto-oncogenes consists of three members: A-raf-1, B-raf, and c-raf-1 which show the greatest degree of homology in the carboxy-terminal half of the molecule. In the case of c-raf-1 this region has been shown to have protein (serine/threonine) kinase activity. C-raf was first identified in an oncogeneic form, v-raf, which is the oncogene of the transforming virus MSV. The raf-1 gene is the most studied of the three and encodes a protein with an apparent molecular mass of∽74kDa. Raf is widely expressed and plays an important role in cell proliferation and differentiation. Raf relays signals related to the activation of growth factor receptor to the activation of MAPK and utlimately to the nucleus. Activation of raf itself is dependent on tyrosine (341) phosphorylation. Tyrosine phosphorylation is accomplished by Src tyrosine kinase.

Specifications

• Antigen: PhosphoDetect™ c-Raf (pTyr³⁴⁰, Tyr³⁴¹)
• Applications: Immunocytochemistry
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: In phosphate buffer (without Mg²⁺ and Ca²⁺), 1mg/mL BSA, 50% glycerol, pH 7.3 (+/- 0.1).
• Host Species: Rabbit
• Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
• Quantity: 10 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes c-Raf phosphorylated at Tyr³⁴⁰ and Tyr³⁴¹ in HEK293 cells overexpressing c-Raf.
• Target Species: Human, Mouse, Rat
• Content And Storage: −20°C
• Form: Purified
• Isotype: IgG

For Research Use Only