MilliporeSigma™ anti-PhosphoDetect Cdk1 (pTyr15), Polyclonal

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 219440100UL

Catalog No. 21-944-0100UL

Description

Specifically detects PhosphoDetect™ Cdk1 (pTyr¹⁵) Clone: in Human, Rat samples, and it is validated for Immunocytochemistry, Immunoprecipitation, Western Blotting

Entry of all eukaryotic cells into M-phase of the cell cycle is regulated by activation of Cdk1 kinase. Activation of Cdk1 is controlled at several steps including cyclin binding and phosphorylation of threonine161. However, the critical regulatory step in activating Cdk1 during progression into mitosis appears to be dephosphorylation of Tyr¹⁵ and Thr¹⁴. Phosphorylation at Tyr¹⁵ and inhibition of Cdk1 is carried out by WEE1 and MIK protein kinases while Tyr¹⁵ dephosphorylation and activation of Cdk1 is carried out by the Cdc25 phosphatase.

Specifications

• Antigen: PhosphoDetect™ Cdk1 (pTyr¹⁵)
• Classification: Polyclonal
• Formulation: In 150mM NaCl, 10mM sodium HEPES, 100mg/mL BSA, 50% glycerol, pH 7.5.
• Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁵ phosphorylation site of human Cdk1
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽34kDa Cdk1 protein, phosphorylated at Tyr¹⁵, in hydroxyurea treated SaoS cell extracts. Also recognizes phosphorylated Cdk2. Does not react with Cdk4, Cdk6, Cdk7, or other phosphotyrosine proteins.
• Form: Purified

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Isotype: IgG

For Research Use Only