MilliporeSigma™ anti-PhosphoDetect EGFR (pTyr1068), Polyclonal

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 32486710T

Catalog No. 32-486-710T

Description

Specifically detects PhosphoDetect™ EGFR (pTyr¹⁰⁶⁸) Clone: in Chicken, Human, Mouse, Rat samples, and it is validated for Immuno Blotting

Epidermal Growth Factor (EGF) is responsible for a variety of biological effects including mitogenesis and differentiation. The EGF receptor (EGFR) has three domains: the extracellular, transmembrane, and cytoplasmic domains. Upon binding of EGF to the extracellular domain, dimerization of the receptor occurs and the cytoplasmic domains display tyrosine kinnase activity. This leads to autophosphorylation of the∽170kDa glycoprotein on various residues within the cytoplasmic domain as well as phosphorylation of intracellular substrates. Tyr¹⁰⁶⁸ is an autophosphorylation site in EGFR. The SH2 domain of Grb2 binds to Tyr¹⁰⁶⁸ of EGFR in the multimeric complex EGFR-Grb2-mSos1 that is formed upon stimulation of EGFR with EGF.

Specifications

• Antigen: PhosphoDetect™ EGFR (pTyr¹⁰⁶⁸)
• Classification: Polyclonal
• Formulation: In Dulbecco's PBS, 1mg/mL BSA, 50% glycerol, pH 7.4.
• Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽185kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
• Content And Storage: −20°C
• Isotype: IgG

• Applications: Immunoblot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Quantity: 10 Tests
• Primary or Secondary: Primary
• Target Species: Chicken, Human, Mouse, Rat
• Form: Purified

For Research Use Only