MilliporeSigma™ anti-PhosphoDetect EGFR (pTyr845), Polyclonal

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 32486510T

Catalog No. 32-486-510T

Description

Specifically detects PhosphoDetect™ EGFR (pTyr⁸⁴⁵) Clone: in Human samples, and it is validated for Immuno Blotting

Epidermal Growth Factor (EGF) is responsible for a variety of biological effects including mitogenesis and differentiation. The EGF receptor (EGFR) has three domains: the extracellular, transmembrane, and cytoplasmic domain. Upon the binding of EGF to the extracellular domain, dimerization of the receptor occurs and the cytoplasmic domain displays tyrosine kinase activity. This leads to the autophosphorylation of the∽170kDa glycoprotein on various residues within the cytoplasmic domain as well as the phosphorylation of intracellular substrates. Tyr⁸⁴⁵ is an autophosphorylation site within EGFR. Phosphorylation of tyrosine 845 is mediated by non-receptor tyrosine kinase c-Src.

Specifications

• Antigen: PhosphoDetect™ EGFR (pTyr⁸⁴⁵)
• Classification: Polyclonal
• Formulation: In Dulbecco's PBS (w/o Mg²⁺ and Ca²⁺), 1mg/mL BSA, 50% glycerol, pH 7.4.
• Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽170kDa EGFR protein phosphorylated at Tyr⁸⁴⁵ in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
• Content And Storage: ≤ -70°C
• Isotype: IgG

• Applications: Immunoblot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Quantity: 10 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only