MilliporeSigma™ anti-PhosphoDetect Neurofilament H Clone: SMI-31,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ NE1022100UL

Catalog No. NE1022100UL

Description

Specifically detects Neurofilament H Clone: SMI-31 in Chicken, Xenopus samples, and it is validated for ELISA, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immuno Blotting

Neurofilaments are a type of intermediate filament that serve as major constituent of the cytoskeleton of axons. They are the most abundant fibrillar components of the axon and are composed of three intertwined protofibrils. The neurofilament triplet proteins (NF-L, 68/70kDa; NF-M, 160kDa; and NF-H, 200kDa) are found in both the central and peripheral nervous system and are usually neuron-specific. NF-H and NF-M both require the presence of NF-L protein to co-assemble. NF-H and NF-M become highly phosphorylated after newly formed neurofilaments enter the axon. Neurofilament staining is observed in neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas, and have also been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung are also known to express neurofilaments.

Specifications

• Antigen: Neurofilament H
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Unpurified
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽180-200kDa phosphorylated neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations. Also weakly recognizes phosphorylated NF-M protein.
• Content And Storage: −20°C

• Applications: ELISA, Immunoblot, Immunoblot, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: SMI-31
• Formulation: Undiluted ascites.
• Immunogen: homogenized, hypothalami from Fischer 344 rat brain
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Chicken, Xenopus species
• Isotype: IgG1

For Research Use Only