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MilliporeSigma™ anti-PhosphoDetect PDH-E1alpha (pSer293), Polyclonal

Catalog No. AP106250UL Shop All MilliporeSigma Products
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Quantity:
50 μL
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
AP106250UL 50 μL Unconjugated
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Catalog No. AP106250UL Supplier MilliporeSigma™ Supplier No. AP106250UL
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Description

Rabbit Polyclonal Antibody

Specifically detects PhosphoDetect™ PDH-E1α (pSer293) Clone: in Human, Mouse, Primate, Rat samples, and it is validated for Immunocytochemistry, Immunoprecipitation

Pyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle. Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases. The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP]). The activity of the PDC complex is mainly regulated by phosphorylation-dehosphorylation of three specific serine residues (232, 293, 300) on the E1 subunit. Phosphorylation of PDH determines the activity of the complex. Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex. The activity of the PDP1 isoform is sensitive to Ca2+, while the PDK isoforms are regulated by the reduction and acetylation status of the E2 subunit, pyruvate, and the small molecule the anti cancer dichloroacetate. (DCA).
TRUSTED_SUSTAINABILITY

Specifications

Antigen PhosphoDetect™ PDH-E1α (pSer293)
Applications Immunocytochemistry, Immunoprecipitation
Classification Polyclonal
Conjugate Unconjugated
Formulation In 150mM NaCl, 100mM Tris-Glycine, pH 7.4.
Host Species Rabbit
Immunogen a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
Quantity 50 μL
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Recognizes the ∽44kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells.
Target Species Human, Mouse, Primate, Rat
Content And Storage −20°C
Form Purified
Isotype IgG
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For Research Use Only

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