MilliporeSigma™ anti-PhosphoDetect Shc/p66 (pSer36) Clone: 60000000000,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 5668071SET

Catalog No. 56-680-71SET

Description

Specifically detects Shc/p66 (pSer³⁶) Clone: 60000000000 in Human, Mouse samples, and it is validated for ELISA, Immuno Blotting

Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: Shc/p46, Shc/p52 and Shc/p66. Shc/p46 and Shc/p52 are found in every cell type, whereas Shc/p66 expression varies in different tissues. Shc proteins are phosphorylated by receptor tyrosine kinases and interact with phosphotyrosine residues on these receptors via the shc PTB and/or SH2 domains. The major tyrosine phosphorylation sites are tyrosines 239/240 as well as tyrosine 317. The Shc/p66 protein contains an additional N-terminal protein domain that is phosphorylated at serine 36. It is suggested that only the serine-phosphorylated Shc/p66 can interact with GRB2. Moreover, expression of Shc/p66 accelerates the inactivation of MAP kinases after EGF stimulation, whereas Shc/p46 and Shc/p52 enhance MAP kinase activity.

Specifications

• Antigen: Shc/p66 (pSer³⁶)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 1 Set
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Form: Purified

• Applications: ELISA, Immunoblot, Immunoblot
• Clone: 60000000000
• Formulation: 100μg antibody lyophilized from 2X PBS, PEG, sucrose and 125μL control protein lyophilized from 2X PBS.
• Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁶ phosphorylation site of human Shc/p66
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽66kDa form of Shc phosphorylated at Ser³⁶. Does not cross-react with the non-phosphorylated form of Shc/p66 or with unrelated phosphorylation sites.
• Content And Storage: −20°C
• Isotype: IgG1

For Research Use Only