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MilliporeSigma™ anti-PhosphoDetect™ Smad3 (pSer423/425) Clone: C25A9, Rabbit Monoclonal,

Rabbit Monoclonal Antibody

Supplier:  MilliporeSigma™ PS102350UL

Catalog No. PS102350UL


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Description

Description

Specifically detects PhosphoDetect™ Smad3 (pSer423/425) Clone: C25A9 in Human samples, and it is validated for Immunoprecipitation

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface to the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at carboxyl terminal serine residues (Ser465/467 on Smad2; Ser423/425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4 and translocate to the nucleus to regulate gene expression.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

PhosphoDetect™ Smad3 (pSer423/425)
Monoclonal
Unconjugated
Rabbit
50 μL
Primary
Human
Purified
Immunoprecipitation
C25A9
In 150mM NaCl, 10mM HEPES, 100μg/ml BSA, 50% glycerol pH 7.5.
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH.
RUO
Recognizes the ∽52kDa Smad3 protein phosphorylated at Ser423/425 in HT-1080 cells treated with TGF-β1.
−20°C
IgG
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