MilliporeSigma™ anti-PhosphoDetect VEGFR2 (pTyr1054/1059), Polyclonal

Catalog No.	Quantity	Conjugate
PS101310T	10 Tests	Unconjugated

Catalog No. PS101310T Supplier MilliporeSigma™ Supplier No. PS101310T

Description

Rabbit Polyclonal Antibody

Specifically detects PhosphoDetect™ VEGFR2 (pTyr^1054/1059) Clone: in Mouse samples, and it is validated for Immuno Blotting

Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) is receptor tyrosine kinase that serves as a key mediator in angiogenesis and plays an essential role in embryonic development, wound healing, cell proliferation, migration, and differentiation. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 (FMS-like tyrosine kinase 1) and Flt-4 (FMS-like tyrosine kinase 4). Upon binding to its ligand, VEGF, VEGFR2 undergoes ligand-induced dimerization, which activates its intrinsic tyrosine kinase activity resulting in auto- and trans-phosphorylation on specific tyrosine residues in the cytoplasmic domain. These tyrosine residues, when phosphorylated, are involved as docking sites to recruit molecules containing Src homology 2 (SH2) or phosphotyrosine-binding domains and to convey signals to downstream effectors. Phosphorylation of VEGFR2 at Tyr¹⁰⁵⁴ and Tyr¹⁰⁵⁹ within the activation loop is required for required for activation of VEGFR2 and enhances its intrinsic tyrosine kinase activity. Phosphorylation at Tyr¹⁰⁵⁹ is also responsible for VEGF-induced intracellular Ca²⁺ mobilization.

Specifications

• Antigen: PhosphoDetect™ VEGFR2 (pTyr^1054/1059)
• Applications: Immunoblot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: In Dulbecco's PBS (without Mg²⁺ and Ca²⁺), 1mg/mL BSA, 50% glycerol, pH 7.3 (± 0.1).
• Host Species: Rabbit
• Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^1054/1059 phosphorylation sites of mouse and human VEGFR-2
• Quantity: 10 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽200kDa VEGFR-2 protein phosphorylated at Tyr^1054/1059 in porcine pancreatic cells transfected with a CSF-1/mouse VEGFR-2 chimeric receptor.
• Target Species: Mouse
• Content And Storage: −20°C
• Form: Purified
• Isotype: IgG

For Research Use Only