PKA[C] Mouse, Unlabeled, Clone: 5B, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 610981

Catalog No. BDB610981

Description

cAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIα, RIβ, RIIα, and RIIβ. These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Cα, Cβ, or Cγ). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. The levels of expression of the different subunits vary according to cell and tissue type.

Immunofluorescence, Western Blotting

Specifications

• Antigen: PKA[C]
• Clone: 5B
• Conjugate: Unconjugated
• Format: Affinity Purified
• Immunogen: Human PKA[Cα] subunit aa. 18-347
• Quantity: 150μg
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Canine, Human, Murine, Rat

• Applications: Western Blot
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Host Species: Mouse
• Isotype: IgG_2b
• Regulatory Status: RUO
• Storage Requirements: Store undiluted at -20°C.
• Monoclonal or Polyclonal: Monoclonal

For Research Use Only.